Notes, summaries, assignments, exams, and problems for Biology

Circulatory System

Blood is a viscous, red, and saline liquid (approximately 5 liters). It is composed of:

• Plasma (55%): A yellowish liquid made of water and substances such as nutrients and hormones.
• Blood Cells: Produced in the bone marrow, there are three types:
  • Red blood cells (erythrocytes): Transport oxygen from the lungs to the cells using a red protein called hemoglobin.
  • White blood cells (leukocytes): Essential components of the immune system.
  • Platelets: Fragments of cells that function to stop bleeding.

Blood Functions

• Transport nutrients
• Transport oxygen and carbon dioxide
• Transport waste products
• Regulate body temperature
• Participate in the immune system
• Prevent hemorrhages

Blood Vessels

• Arteries: Transport blood from the heart to the organs.

Cancer Stem Cells vs. Normal Stem Cells

Both normal stem cells and cancer stem cells (CSCs) share the ability to self-renew through asymmetric division, allowing one daughter cell to remain a stem cell while the other differentiates. They both generate heterogeneous populations of mature cells: normal stem cells produce tissue-specific cell types, while CSCs produce diverse tumor cells. Both rely on similar regulatory signaling pathways such as Wnt, Notch, and Hedgehog, but these pathways are tightly controlled in normal stem cells and dysregulated in CSCs, promoting tumor formation. Both exist as rare subpopulations within tissues, depend on signals from their niche, and can remain quiescent for long periods. CSCs, however, show enhanced resistance... Continue reading "Cancer Stem Cells: Signaling Pathways and Therapeutic Potential" »

Animal Development and Embryology

• Which best describes the blastula development stage? - A hollow ball of cells with a blastocoel.
• In early stages of development through the gastrula stage: Cells divide to increase the number of cells but they don’t increase in size.
• A fertilized egg cell is: Diploid.
• Which best describes the zygote development stage? - A fertilized egg cell.
• Which of the following correctly displays the sequence of developmental stages? - Cleavage-blastula-gastrula.
• The first cavity in an embryo formed during frog development is the: Blastocoel.
• The archenteron of the developing sea urchin eventually develops into the: Digestive tract or gut.
• _____ has determinate cleavage and will not have identical twins: Protostome.
• ____ has the

Lab 8: Temperature and Microbial Growth

Key Concepts

• Psychrophiles grow best between 0–20°C. Often found in cold environments like glaciers.
• Mesophiles prefer 20–45°C. These include human pathogens like E. coli.
• Thermophiles thrive at 55°C or higher. They are often found in hot springs.

Materials and Their Functions

• Tryptic Soy Broth (TSB): A nutrient-rich medium that supports bacterial growth.
• Incubators set to different temperatures (4°C, 25°C, 37°C, 55°C): Used to simulate environmental conditions.
• Spectrophotometer (OD600): Measures turbidity (cloudiness) of a culture to estimate cell density.
• Classification by growth: “+++” indicates heavy growth; “0” means no growth.

In this lab, students investigated how temperature affects... Continue reading "Microbiology Lab Fundamentals: Growth, Metabolism, Genetics" »

Plant Anatomy and Reproduction

The flower, which is responsible for reproduction, has a female part: the carpel, and a male part: the stamen. The female gametes (ovules) are produced in the ovary inside the carpel, while the male gametes are called pollen grains. Petals attract animals and protect the flower, while sepals cover and protect the other parts of the flower.

As a result of fertilization, a seed develops, which is responsible for the survival and dispersal of the plant.

3. Reproduction in Plants

Aquatic organisms often use water as a medium for transporting their gametes and for fertilization. On land, the vast majority of plants have developed mechanisms of reproduction that make their fertilization independent of water. Plants can... Continue reading "Plant Reproduction and Photosynthesis Explained" »

Nitrogen Fixation and Symbiosis

Module 3: N₂ + 8e^- + 8H⁺ + 16ATP → 2NH₃ + H₂. Nitrogenase is killed by O₂; leghemoglobin binds O₂ (pink), nodules block O₂, and high respiration lowers free O₂. The plant provides sugars while rhizobia fix N₂. High N levels lead to glutamate, while low N levels lead to glutamine. Plant flavonoids trigger bacterial Nod factors (LPS signals) to initiate symbiosis. Root hair curls (“shepherd’s crook”) trap bacteria and start nodule formation.

Archaea: Biology and Genomics

Archaea: Once thought to be only extremophiles, they are actually ubiquitous. Identified via 16S rRNA (distinct from bacteria and eukaryotes). Methanogens: Only archaea perform methanogenesis, the terminal step of the C-cycle and a source... Continue reading "Microbiology Fundamentals: From Microbial Ecology to Genomics" »

Essential Biological Macromolecules

Properties: Hydrophilic vs. Hydrophobic

• Hydrophilic (water-loving): Nucleic Acids, Carbohydrates, Starch, Chitin. Glucose is hydrophilic because it contains many hydroxyl functional groups.
• Hydrophobic (water-fearing): Lipids.

Key Molecules and Building Blocks

• Organic Molecules: Examples include Methane (CH4) and Glucose.
• Creating Polymers: Polymers are created from monomers through a Dehydration Reaction.

Types of Macromolecules

Carbohydrates

• Monosaccharides: Simple sugars like glucose, fructose, and galactose.
• Disaccharides: Maltose is formed from two glucose molecules.

Lipids

• Unsaturated Fats & Oils: These contain carbon-carbon double bonds in their fatty acids.

Proteins

• Proteins are unique in that they contain

Microbiology Lab Practice Questions

What is the optimal temperature range for Bacillus stearothermophilus?

Answer: 55°C (a thermophile)

Which organism produces red pigment at 25°C?

Answer: Serratia marcescens

What does no pigment but visible turbidity in S. marcescens at 37°C suggest?

Answer: Growth occurred, but pigment production is temperature-sensitive.

What is the term for bacteria that grow best at cold temperatures but also at room temperature?

Answer: Psychrotroph (e.g., Pseudomonas fluorescens)

What color does E. coli appear on MacConkey agar?

Answer: Pink (lactose fermenter)

Which test detects mixed acid fermentation?

Answer: Methyl Red (MR) test

What does a black precipitate on HE agar indicate?

Answer: H₂S production (e.g., Salmonella)

Which

Clinical Characteristics of Polymyositis

Myopathic Weakness

Myopathic weakness evolves over weeks to months, typically sparing the facial and eye muscles. It presents as difficulty in:

• climbing steps
• rising from a chair
• lifting objects
• combing hair

Disease Onset

Typically occurs above the age of 18 years.

Features the Patient Does Not Have

The following features are not characteristic of polymyositis in the typical presentation:

• Rash (characteristic of dermatomyositis)
• Family history of neuromuscular diseases
• Exposure to myotoxic drugs, especially penicillamine²⁶, zidovudine²⁷, and (rarely) statins^28,29
• Endocrine disease (hypothyroidism, hyperthyroidism, hypoparathyroidism, hypercortisolism)

Excluded Conditions

• Neurogenic disease (excluded by electromyography

Oral Anatomy and Dental Structures

• Mouth (Boca): Another term for the oral cavity.
• Oral Cavity (Cavitat Oral): Another name for the mouth.
• Teeth (Dientes): Hard, white, enamel-covered structures used to chew and bite food.
• Tongue (Lengua): A muscle located at the base of the oral cavity used to speak and eat.
• Tonsils (Amígdalas): Structures located at the left and right sides of the palatoglossal arch.
• Uvula: A cone-shaped tissue that hangs down from the soft palate.
• Hard Palate (Paladar Duro): The bony front portion of the roof of the mouth.
• Soft Palate (Paladares Blandos): A fibrous and muscular soft tissue that comprises one-third of the roof of the mouth.
• Upper Lip (Labio Superior): A structure made of soft, pink tissue close to the nose.
• Lower