Notes, summaries, assignments, exams, and problems for Biology

In Situ Tests: Parameters, Methods, Limitations

In situ tests: Values of different parameters are obtained from theoretical interpretation of the test result. Compared with laboratory tests, in situ tests reduce some limitations such as:

• Disturbance of soil samples (matters less; no transport of the sample).
• Change in the stress state of the sample.
• Small size of laboratory samples may be unrepresentative (a larger portion of the soil is involved).

Other limitations appear:

• Some disturbance is always present.
• Less control over test conditions (in the laboratory we can control water conditions).
• If the parameters of the theoretical model are two or more, only a relationship between them is obtained (for example, E and ν, or c and φ).

Common In Situ

Nutritional Needs

D(calciferol); it helps the calcification of our bones, it is present in milk and dairy products, fish liver oil. C(ascorbic acid), antioxidant and it keeps mucus normal and healthy, it is present in citrus fruits, strawberries, and vegetables.

Structural

The most important structural nutrients are proteins, although certain lipids, cell membranes, and certain salts such as the ones that form our skeleton.

Energy Needs

Our body needs energy to perform any physical activity. Energy nutrients (carbohydrates and fats) can be obtained from proteins, this process is possible due to cell respiration.

Different Caloric Values

One gram of fat 9 kcal, one gram of carbohydrates 3.75 kcal, one gram of protein 4 kcal.

Basal Metabolic Rate and

Cooking Techniques

Moist-Heat Techniques

Methods in which heat is conducted to foods by water or other liquid or by steam:

• Simmer: To cook in water or other liquid that is bubbling gently (85-96°C). Similar to poaching, the only difference is the time.
• Steam: To cook by direct contact with steam.
• Boil: To cook in water or other liquid that is bubbling rapidly, about 100°C at sea level and at normal pressure.
• Poach: To cook gently in water or other liquid that is hot but not actually bubbling (71-82°C).
• Blanch: To cook an item partially and very briefly in boiling water or in hot fat. Usually a pre-preparation technique, as to loosen peels of vegetables, fruits, and nuts, to partially cook French fries or other foods before service, to prepare for

Human Receptors and Nervous System

Receptors: Structures that human beings use to detect stimuli.

Interoceptors

Collect information about the body’s internal conditions. Located in the internal environment.

Exteroceptors

Provide information from the body’s outside environment. Located on the surface of the body.

Proprioceptors

Inform about the relative position and condition of each of the body’s muscles. Found in the inner ear’s vestibule and locomotive system.

Eyes/Sight

Detect light stimuli. Photoreceptors

Ears/Hearing

Detect sound waves (Mechanoreceptors) and proprioception.

Nose/Smell

Detect substances dissolved in the air. Chemoreceptors

Mouth/Taste

Detects substances dissolved in liquids found in the oral cavity. Chemoreceptors

Skin/Touch

Detects... Continue reading "Human Receptors and Nervous System: An Overview" »

1. Telomerase is a ribonucleoprotein complex that consists of telomerase RNA (which contains several tandem hexanucleotide repeats complementary to the telomere repeats in the substrate) and a reverse transcriptase. This complex is able to synthesize telomeric repetitive DNA sequences at the end of linear DNA molecules. Telomerase RNA binds to the DNA strand, and complementary dNTPs are incorporated into newly synthesized, extended DNA strands by reverse transcriptase. Thus, dNTPs must also have been present during step 1 of the TRAP assay (step 1).

2. The PCR reaction mixture also contained a heat-resistant DNA polymerase (Taq polymerase).

3. Sample 3 contained cell extract isolated from immortal cells. In the presence of the telomerase, products... Continue reading "Telomerase Activity in Immortalized Cells" »

Blood Composition and Functions

Cells live immersed in a medium that provides them with all the nutrients they need. They also secrete the waste products released during metabolism into this medium. In multicellular organisms like humans, this medium is called the internal milieu and is made up of the interstitial plasma, a fluid found in the space between the cells. The interstitial plasma is renewed thanks to the blood, which continuously circulates around the whole body, providing nutrients and taking away waste products.

What is Blood?

Blood is a viscous fluid with a salty taste that flows inside the vessels that make up the circulatory system.

Blood Components

Blood is made up of cells suspended in a liquid called blood plasma.

• Blood plasma

Understanding Cells: Structure, Functions, and Types

The cell is the smallest unit of a living organism that can carry out vital functions: nutrition, interaction, and reproduction.

Essential Cell Components

All cells possess a plasma membrane, cytoplasm, and DNA.

• Plasma Membrane: This is a very thin and stretchy cover that separates a cell from its environment. Its primary function is to regulate the movement of substances in and out of the cell. It also detects environmental stimuli and allows cells to communicate.
• Cytoplasm: This is the substance contained inside the cell, in which the cell organelles are found. Metabolic processes occur within the cytoplasm.
• Genetic Material (DNA): A complex chemical substance that contains the information needed

Oparin’s Theory of the Origin of Life

1. The primitive atmosphere was made up of gases: ammonia, water vapor, methane, and hydrogen.
2. The energy for the first chemical reactions to occur came from volcanic eruptions, radiation from the sun, and storms.
3. The temperature of the Earth fell, so the water vapor condensed and formed seas.
4. Organic molecules, synthesized by the reactions, accumulated in the water and joined together to form bigger molecules.
5. Coacervates were structures formed by organic molecules insulated from the water.
6. The first cell appeared when the coacervates started to reproduce.

The Endosymbiotic Theory

The Endosymbiotic Theory of Margulis explains the appearance of the first eukaryotic living things from the symbiotic relationships... Continue reading "Origins of Life and Biological Evidence for Evolution" »

Skeletal System: Functions and Joints

Skeletal System Functions

• Support the body structure.
• Protect vital organs.
• Provide points of attachment for muscles.
• Manufacture blood cells (hematopoiesis).
• Store calcium and phosphorus.

Joints: Connections Between Bones

Joints are places where bones connect. They are classified by the degree of movement they permit:

• Fixed Joints (Synarthroses): Do not permit movement (e.g., skull sutures).
• Slightly Movable Joints (Amphiarthroses): Allow limited movement (e.g., vertebrae).
• Freely Movable Joints (Diarthroses): Allow bone movement in all directions (e.g., knee).

Sensory Receptors and Stimuli Perception

Types of Receptors

• Internal Receptors (Interoceptors): Perceive changes occurring inside the body (e.g., blood pressure,