Principles of Spectrophotometry and Radiant Energy Interaction
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Color and the Visible Spectrum
The eye can discern the visible spectrum.
Spectrometry and Spectrophotometry
In spectrometry and spectrophotometry, the energy incident on a sample is monochromatic radiation (radiant energy of a single wavelength or, for practical reasons, a very narrow band of wavelengths). The transmitted radiation is measured by highly sensitive devices such as photomultiplier tubes, photocells, and thermocouples.
Interaction Between Radiant Energy and Matter
Molecules, ions, or atoms can absorb radiation if the energy of the photons matches the natural frequency of vibration of electrons and/or atoms in molecules. Photons of different energies produce distinct effects on the absorbing material:
- Gamma and Cosmic Rays: Very short