pBR322: The First Artificial Cloning Vector
Classified in Biology
Written on in
English with a size of 73.57 KB
pBR322 was the first artificial cloning vector created in the laboratory by Francisco Bolivar and Raymond L. Rodriguez. Compared to its pUC counterparts, pBR322 is larger in size, resulting in a slower rate of replication. This E. coli cloning vector contains an origin of replication (ori), restriction sites, and antibiotic-resistant genes.
pBR322 is a widely used cloning vector in E. coli with significant applications in molecular cloning.
pBR322 Nomenclature
- p: Plasmid
- BR: Bolivar and Rodriguez
- 322: Numerical designation
Constructed in 1977 in the laboratory of Herbert Boyer at the University of California, San Francisco, it was the first synthetic plasmid designed for use as a cloning vector. As one of the most studied plasmids, it is 4,362 base... Continue reading "pBR322: The First Artificial Cloning Vector" »