Microbiology Lab Techniques and Experiments
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Genetic Material/DNA
Variable Cell Count
- DNA must contain biologically useful information.
- Must be capable of reproduction = replication = growth.
- Must be decodable (MRNA) transcription/translation.
- Must be able to change or show variation (mutate/adapt).
H20 Sample -> 1ml -> A10(2) -> 1ml -> B10(4) -> 1ml -> C10(6)
0.1ml D10(5) F10(7)
1ml E10(4) G10(6)
- Pour 10ml cooled molten agar over the bottom of the plate, close the lid, swirl to mix and allow to solidify.
- Invert & incubate at 37C for 24-48h.
- After incubation, observe.
- Select a plate with 30-300 colonies.
- Count all colonies on the agar surface/embedded in agar.
- Colonies X dilution factor = colonies/ml
Griffith's Experiment
- Injected living S in mouse - mouse died.
- Injected