Clinical Laboratory Fundamentals: Instruments, Assays, Values
Classified in Medicine & Health
Written on in
English with a size of 5.01 KB
Clinical Laboratory Equipment Essentials
Water Bath
Used to incubate samples at a desired temperature.
Centrifuge
An equipment used to separate substances that differ significantly in their masses by using centrifugal force.
Spectrophotometer
Measures substances in the ultraviolet (200 – 400 nm) as well as in the visible range (400 – 700 nm) of the electromagnetic spectrum of light.
Micropipettes
Used to dispense micro volumes of samples or reagents.
Enzyme Kinetics and Inhibition
Competitive vs. Non-Competitive Inhibitors
| Parameter | Competitive Inhibitor | Non-Competitive Inhibitor |
|---|---|---|
| Km | Increase | No Change |
| Vmax | No Change | Decrease |
Relationship Between Km and Substrate Affinity
- An enzyme with a high Km has a low affinity for its substrate and requires a greater concentration