Understanding Genetic Engineering and Cloning
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Genetic Engineering
Genetic engineering is the process of changing the DNA composition in organisms using laboratory techniques.
Obtaining Recombinant DNA Using Vectors
Recombinant DNA is an artificial molecule obtained from organisms. The stages include:
- Locate the required gene and analyze its nucleotide sequence.
- Isolate the gene with enzymes that cut DNA into specific sections.
- Connect the gene to a transportation vector, such as virus DNA or bacteria. The combination of the gene and vector results in recombinant DNA.
- Introduce the recombinant DNA into the cell.
Polymerase Chain Reaction (PCR) Technique
- Heat DNA to separate the strands.
- Add DNA polymerase enzyme and nucleotides to the tube to synthesize complementary strands.
- Separate the formed strands