Polarimetry and Refractive Index Measurement Techniques
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Abbe Operation
Abbe Operation is based on the determination of the critical angle. The technique is to calibrate the device, usually with distilled water at 20ºC, by matching the shade formed by the prism surface illuminated by a brand (not recorded) in the center of the telescope. Perform the same operation with the test sample to make the correct reading of the refractive index in the eyepiece of the telescope. Since the refractive index varies with temperature, it is important to perform the measurement with the apparatus thermostatted at 20°C or at least know the temperature at which to make the determination.
Understanding Light Polarization
- Natural Light: It is a vibration in all directions perpendicular to the beam.
- Polarized Light: It