Understanding Chromatography: Techniques and Applications
Principle of Chromatography - Chromatography is a separation method where the analyte is combined within a liquid or gaseous mobile phase, which is pumped through a stationary phase. Usually, one phase is hydrophilic and one is lipophilic. The components of the analyte interact differently with these two phases. Depending on their polarity, they spend more or less time interacting with the stationary phase. This leads to the separation of different components present in the sample. Each sample component elutes from the stationary phase at a specific time called retention time.
Types of Chromatography
- Liquid Chromatography - This type of chromatography is used to separate and analyze non-volatile compounds. In this technique, the sample is dissolved
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