Essential Oil Separation and TLC Analysis Techniques
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Essential Oil Chromatography and Chemical Properties
This document summarizes key chemical data and procedures related to the chromatographic separation and analysis of essential oils, focusing on techniques like Thin-Layer Chromatography (TLC).
Aim of the Chromatographic Exercise
The primary objective of this laboratory exercise is:
- Chromatographic division of essential oils using two-way TLC chromatography.
Essential Oil Composition
Essential oils are complex mixtures of compounds that belong to several major chemical classes. These include:
- Terpenes
- Sesquiterpenes
- Aldehydes
- Alcohols
- Ketones
- Acids
Reagents and Solvents for Analysis
Extraction Reagent for Azulene Oils
The specific reagent used to extract oils containing azulene is:
Benzene
Chromatographic Solvent for Raphacholin (One Direction)
The solvent system used for the one-directional TLC analysis of Raphacholin is composed of:
Mixture Ratio: Ethyl acetate (1) : Benzene (5)
Thin-Layer Chromatography (TLC) Results
During the TLC procedure, the characteristic color observed for the azulene stain is:
Red