Reverse Transcriptase and Gene Therapy in Molecular Biology
Classified in Biology
Written on in
English with a size of 1.79 KB
Reverse Transcriptase
Production of DNA from RNA
Reverse transcriptase is an enzyme isolated from retroviruses that catalyzes the production of DNA from an RNA template.
Use in Molecular Biology
- Reverse transcriptase is used in recombinant DNA technology to produce genes for gene transfer.
- It catalyzes the production of complementary DNA (cDNA) from an mRNA template.
- cDNA does not contain introns because mRNA undergoes splicing before cDNA production.
- Bacteria lack the machinery for intron removal, so genes inserted into them need introns removed to produce functional proteins.
- Examples of reverse transcriptase use include:
- Mass production of human insulin by E. coli.
- Generation of cDNA libraries for DNA microarrays (DNA fingerprinting).