Histology Tissue Preparation: A Step-by-Step Protocol
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1. Sampling
Taking a tissue sample quickly and without traumatizing it.
2. Fixation
The sample is placed in fixatives, such as formalin, Zenker's solution, or Bouin's solution (the most commonly used). After fixation, the tissue changes color.
3. Dehydration and Clearing
First, remove excess fixative with water. Then, the tissue is dehydrated through a series of alcohol or acetone baths. The sample is immersed in increasing concentrations of alcohol: 50%, 70%, 96%, and finally 100% (absolute alcohol). This process typically takes 30 minutes.
After dehydration in alcohol, the sample is immersed in Methyl Benzoate I, where it initially floats and then sinks. Finally, it is submerged in Methyl Benzoate II. The clearing agent, Xylene, is then used for... Continue reading "Histology Tissue Preparation: A Step-by-Step Protocol" »