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Histology Tissue Preparation: A Step-by-Step Protocol

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1. Sampling

Taking a tissue sample quickly and without traumatizing it.

2. Fixation

The sample is placed in fixatives, such as formalin, Zenker's solution, or Bouin's solution (the most commonly used). After fixation, the tissue changes color.

3. Dehydration and Clearing

First, remove excess fixative with water. Then, the tissue is dehydrated through a series of alcohol or acetone baths. The sample is immersed in increasing concentrations of alcohol: 50%, 70%, 96%, and finally 100% (absolute alcohol). This process typically takes 30 minutes.

After dehydration in alcohol, the sample is immersed in Methyl Benzoate I, where it initially floats and then sinks. Finally, it is submerged in Methyl Benzoate II. The clearing agent, Xylene, is then used for... Continue reading "Histology Tissue Preparation: A Step-by-Step Protocol" »

Firefighting Foams, Dry Columns, and Fire Types

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Firefighting Foams Characteristics

  • Fluency and Sealing: Ensures quick and effective surface coverage, preventing fuel vapor escape.
  • Heat Resistance: Maintains cooling properties under mechanical stress and heat.
  • Durability: Resists heat, flames, and wind; regenerates if surface cracks occur.
  • Contamination Resistance: Maintains a uniform bubble structure when exposed to liquid fuels.
  • Thickness: Holds enough water to withstand contact with hot surfaces of non-liquid or solid fuel.
  • Oxygen Separation: Physically separates oxygen from the air above the ignited fuel surface.
  • Heat Absorption: Absorbs heat from the fuel and adjacent solid surfaces.
  • Radiation Prevention: Prevents heat transmission from flames via radiation.

Dry Columns: Function and Operation

A... Continue reading "Firefighting Foams, Dry Columns, and Fire Types" »

Understanding Micrometers, Melting Points, and Fluid Properties

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Error Variables in Micrometers

Error variables are errors that vary according to the dimension being measured. Such errors mainly come from variations in the passage of the screw micrometer. It is not possible to correct a variable error in the micrometer, but the error can be known if the tool has it.

Other Types of Micrometers

By suppressing, eliminating, or modifying the body of a standard micrometer, variants can be obtained. Different models are set up to fit a wide variety of measurements, including:

  • Interior micrometer
  • Micrometers with three inner supports
  • Interior micrometers for small diameters
  • Depth micrometers
  • Special micrometers

The Melting Point

The melting point is a physical constant used in organic machines, but it can be defined as the... Continue reading "Understanding Micrometers, Melting Points, and Fluid Properties" »

Understanding the Periodic Table and Chemical Bonds

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Periodic Table of Elements

7s 7p
6s 6p 6d
5s 5p 5d 5f
4s 4p 4d 4f
3s 3p 3d
2s 2p
1s

Elements are sorted by atomic number and grouped by chemical and physical properties, with 18 groups and 7 periods.

Group

A group consists of elements that have a similar electron configuration at the highest energy level. They have the same number of electrons in the same type of sub-levels, although at different energy levels. The chemical characteristics are similar and depend on the outermost electrons of the atoms. There are 18 groups.

Period

A period comprises all elements whose maximum energy level is the same. There are 7 periods: the 1st has 2 elements, the 2nd and 3rd have 8 elements, the 4th and 5th have 18 elements, and the 6th has 32 elements.

Periodic

... Continue reading "Understanding the Periodic Table and Chemical Bonds" »

Understanding Fundamental Chemistry Terms

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Fundamental Chemistry Concepts

Mixtures: Homogeneous vs. Heterogeneous

Homogeneous means it has a uniform appearance; at first glance, there are no distinct components. A heterogeneous mixture consists of several substances that are visibly separate.

Understanding Solutions

A solution is a homogeneous mixture, consisting of two or more pure substances present in varying amounts. These components can be separated by physical processes.

Solution Components: Solvent and Solute

Normally, the substance found in the largest quantity is called the solvent. The other substances are called solutes.

Solution Concentration: Concentrated/Diluted

A solution is concentrated when it contains a large amount of solute. A solution is dilute when it contains a small... Continue reading "Understanding Fundamental Chemistry Terms" »

Mineralogy and Crystallography: Key Concepts and Properties

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Mineralogy and Crystallography

1. Define Polymorphism

Polymorphism refers to the phenomenon where the same chemical substance can exhibit different physical and even chemical properties due to variations in formation conditions, such as pressure and temperature. These variations lead to different arrangements of atoms within the crystal structure.

2. Define Isomorphism

Isomorphism occurs when two or more chemical compounds adopt the same crystalline structure.

3. Define Pseudomorphs

Pseudomorphs are minerals where the internal structure does not correspond to the external crystal form, resulting in a "false form."

4. Define Solid Solution and Mixing

  • Solid solution: A homogeneous solid with a variable composition.
  • Mixing: A type of solid solution where
... Continue reading "Mineralogy and Crystallography: Key Concepts and Properties" »

Understanding Eye Conditions and Nuclear Physics Principles

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Eye Conditions: Myopia and Hyperopia

Myopia

Myopia occurs when the eye has excessive convergence, focusing light from distant objects in front of the retina, resulting in blurred distant vision. This condition is corrected with diverging lenses.

Hyperopia

Hyperopia occurs when the eye is less convergent than normal, causing images of nearby objects to form behind the retina, leading to blurred near vision. This condition is corrected with converging lenses.

Fundamentals of Nuclear Physics

First Model of the Nuclear Atom

The atom consists of a nucleus, which contains almost all of its mass and all of its positive charge. Electrons orbit the nucleus at some distance. The nuclei are formed by two types of particles called nucleons: protons and neutrons.... Continue reading "Understanding Eye Conditions and Nuclear Physics Principles" »

Copper Extraction: Pyrometallurgy vs. Hydrometallurgy

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Copper Metallurgy: Pyrometallurgy vs. Hydrometallurgy

The metallurgy of copper depends on the mineral ore. Sulfide ores utilize a pyrometallurgical route to produce anodes or cathodes, while oxide ores use a hydrometallurgical route to directly produce cathodes.

Pyrometallurgy

The cathode production process via pyrometallurgy is as follows: Mineral → Smelting (furnace) → Converters → Refining anode casting → Refining → Cathode.

Copper sulfide ore, with a low grade of 0.5%, requires concentration by flotation. The copper concentrate received in the foundry industry undergoes melting in a furnace, where copper is recovered, and sulfur and iron are removed through oxidation. The copper settles at the bottom and exits the furnace as copper... Continue reading "Copper Extraction: Pyrometallurgy vs. Hydrometallurgy" »

Atomic Structure and Chemical Formulas

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Isotopes and Atomic Structure

Isotopes

Atoms of the same element can have different masses but identical chemical properties. These are called isotopes.

The Neutron

Arranging elements by increasing atomic number resolves anomalies in the periodic table that arise when ordering by atomic mass. Isotopic masses, determined by mass spectrometry, are inconsistent with the mass of protons in the atomic number. Therefore, atomic mass must be explained by other factors.

Atomic Number (Z)

This number indicates the number of protons in the nucleus and defines the element. It is represented by the letter Z.

Mass Number (A)

This number indicates the total number of protons and neutrons (nucleons) in the nucleus and defines the isotope of an element. It is represented... Continue reading "Atomic Structure and Chemical Formulas" »

Fundamental Chemical & Biological Concepts

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Elemental Properties & Periodic Trends

Metallic Properties in the Periodic Table

Across a Period

Within the same period, the metallic properties of elements increase as one moves to the left and decrease as one moves towards the right.

Down a Group

Within the same group, the metallic properties of elements increase as one moves down the table and decrease as one moves up.

Essential Elements & Their Occurrence

Bioelements: Building Blocks of Life

Primary Bioelements

These are the most abundant elements in living organisms:

  • Hydrogen (H)
  • Carbon (C)
  • Nitrogen (N)
  • Oxygen (O)
  • Phosphorus (P)
  • Sulfur (S)

Indispensable Secondary Bioelements

These are essential for life, required in smaller amounts:

  • Sodium (Na)
  • Magnesium (Mg)
  • Potassium (K)
  • Calcium (Ca)
  • Manganese (Mn)
... Continue reading "Fundamental Chemical & Biological Concepts" »