Cytology Fixatives and Staining Techniques
Classified in Chemistry
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Cytology Fixatives
Solution: Cytology fixatives, formerly employing ether/alcohol 96 in equal parts, are now rarely used due to the hazardous nature of ether. The 96% alcohol is most often used. The procedure involves immersing the preparation in the fixative bath for a minimum of 10 to 15 minutes. Other alcohols, such as 100% methanol, 80% propanol, and 80% isopropanol, can also be used. Citospray is used in samples obtained by forced exfoliation.
Sample Types in a Cytology Laboratory
Samples that can reach the lab from samples obtained by:
- Forced exfoliation: Rubbing or scraping with various instruments. This is applied to the skin and organs accessible from the outside.
- Spontaneous exfoliation: Samples containing spontaneously exfoliating