Cell Biology: Actin, Myosin, and Microtubules
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Polymerization –plus and minus ends of actin, Kd, critical concentration, phases of growth of polymers (lag, growth, and steady-state), nucleating factors, why growth occurs preferentially at plus end.
a.High Kd and Cc= ADP + Factin lower affinity actin so dissociate
b.Lower kd and Cc= Gactin/Factin + ATP higher affinity actin so dissociate
c.Profilin and thymosin: Profilin binds to (-) end = plus end grow fast
d.Thymosin buffer + actin monomer = prevent polymerization or ATP hydrolysis.
e.Actin monomer (is above Kd) & regulates actin filament assembly.
Arp2/3 comples to make a seed (nucleate) acting growth. Can create tree-like web
Myosin II structure: motor protein makes force in skeletal contraction muscles.
a.Heavy chain with NT head (ATPase... Continue reading "Cell Biology: Actin, Myosin, and Microtubules" »