DNA Transcription: Process, Elements, and Stages
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Elements Involved
In order to perform the transcription of DNA into cells, the following elements are required:
- Original DNA to serve as a template to be copied.
- RNA polymerase: synthesizes RNA from a DNA template.
- Ribonucleotide triphosphates to perform the copy.
- Poly-A polymerase, small nuclear ribonucleoprotein, RNA ligase.
Transcription Mechanism
As in replication, there are differences between prokaryotes and eukaryotes. The main differences are the existence of multiple RNA polymerases in eukaryotes and, above all, the need to produce a "mature" form of some RNAs due to the existence of introns. The process is divided into three stages:
- Initiation: The RNA polymerase binds to a region of DNA prior to the DNA that is to be transcribed. It then