Notes, summaries, assignments, exams, and problems for Biology

Key Concepts in Microbial Pathogenicity

• Pathogenicity: The ability of a microorganism to cause disease.
• Virulence: The extent or degree of pathogenicity.
• Normal Microbiota: Resident microbes, often referred to as commensals.
• Infection: The invasion of normally sterile tissues by microorganisms.
• Pathogen: An infectious agent capable of causing disease.
• Infectious Disease: A disease caused by infection by a pathogen.
• Colonization: The process of establishing a population of organisms in a host.
• Resident Colonizer: An organism that establishes a long-term presence in the host.
• Transient Presence: A short-term presence of an organism in the host.

Major Factors in the Development of an Infection

1. Adherence: Initial Attachment to Host Cells

   Adherence is crucial

The Mechanism of Photosynthesis and Energy Conversion

Photosynthesis (pronounced FOH-tə-SINTH-ə-sis)^[1] is a system of biological processes by which photopigment-bearing autotrophic organisms, such as most plants, algae, and cyanobacteria, convert light energy—typically from sunlight—into the chemical energy necessary to fuel their metabolism. The term photosynthesis usually refers to oxygenic photosynthesis, a process that releases oxygen as a byproduct of water splitting.

Storing Chemical Energy and Maintaining the Atmosphere

Photosynthetic organisms store the converted chemical energy within the bonds of intracellular organic compounds (complex compounds containing carbon), typically carbohydrates like:

• Sugars (mainly glucose, fructose,

Electrical Fundamentals in Electrotherapy

Electrotherapy involves the use of electric currents for therapeutic purposes, commonly used in physical therapy and rehabilitation. Understanding the electrical fundamentals is crucial for effectively applying electrotherapy techniques.

Basic Concepts of Electricity

• Current (I): The flow of electric charge, measured in Amperes (A). It can be direct (DC) or alternating (AC).
• Voltage (V): The electrical potential difference that drives current flow, measured in Volts (V).
• Resistance (R): The opposition to current flow, measured in Ohms (Ω). According to Ohm's Law: V=I×R.

Types of Electric Currents in Electrotherapy

• Direct Current (DC): A unidirectional flow of electric charge, used in applications like iontophoresis.

Physiology Assessment Solutions

Blood Composition and Function

• 1. e. A and C: Lungs and Intestines bring in oxygen and nutrients into the blood.
• 2. d. 92%
• 3. a. Fibrinogen
• 4. c. The heme group
• 5. a. One
• 6. d. Leukocytes: Leukocytes (white blood cells) are not dissolved in plasma; they are cells that circulate or reside in tissues.
• 7. d. Erythropoietin: The hormone that stimulates red blood cell production is erythropoietin.
• 8. a. 250,000,000: Each red blood cell contains approximately 250 million hemoglobin molecules.

Hemostasis and Clotting

• 9. b. Fibrinogen: Thrombin converts fibrinogen into fibrin to form blood clots.
• 10. c. Tissue factor: Tissue factor, exposed upon damage, activates plasma proteins in the clotting cascade.
• 11. d. Plasmin: Plasmin dissolves

Constructing Recombinant DNA

The following steps are involved in the construction of recombinant DNA:

1. Preparation of the Gene: Gene cloning in bacteria is achieved by cleaving DNA with enzymes called restriction endonucleases, which create small fragments. Each fragment often has a "sticky end." Since eukaryotic genes contain introns that are not processed in bacteria, the DNA for cloning is usually obtained from relevant mRNA through the process of reverse transcription. In cases where nucleotide or amino acid sequences are known, synthetic DNA may also be produced.
2. Insertion into a Vector: The vector is a vehicle, such as a plasmid or bacteriophage, used to transfer DNA into a host cell. The vector is cut with the same restriction endonuclease

Direct DNA Transfer: Vectorless Gene Introduction

The term direct or vectorless transfer of DNA is used when foreign DNA is introduced directly into the host genome, bypassing biological vectors.

Key Direct Gene Transfer Methods

1. Electroporation

   Electroporation involves using high field strength electrical impulses to reversibly permeabilize cell membranes, allowing the uptake of DNA. This technique can be used for the delivery of DNA into intact plant cells and protoplasts. Nowadays, intact cells, callus cultures, and immature embryos can be used with suitable pre- and post-electroporation treatments. Electroporation has been successfully employed for producing transgenic plants of many cereals, such as rice, wheat, and maize.

2. Particle Bombardment

Define Callus and Describe Procedure For Callus Culture

A callus culture is a mass of irregular undifferentiated parenchymatous and totipotent cells. These cells have the ability to divide and differentiate to form an entire organism. It is formed in culture media due to active mitotic division of the cells of the explant. Callus culturing is used in the production of secondary metabolites for plant regeneration.

Types of Callus Culture

• Compact Callus
• Friable Callus

1) Compact Callus

It shows densely aggregated cells.

2) Friable Callus

It shows loosely associated cells, and the callus becomes soft and breaks easily.

Stages of Callus Culture

There are three stages of callus culture:

1) Induction

Cells in the explant dedifferentiate and begin to divide.

2)

Biology Concepts

Cellular Processes and Structure

• 1-A) Ev against abiogenesis.
• 3-C) Cannot maggots developed.
• 4-B) Life forms from non-living matter.
• 5-C) Cells contain organelles.
• 6-B) Move through phospholipid bilayer quickly.
• 7-A) Endocytosis.
• 8- Vacuole: 3, Nucleus: 7, Cytoplasm: 2, Rough ER: 4.
• 9- Synthesize lipids: 5, Convert glucose: 8, Convert sunlight: 1, Modify lipids: 6.
• 10-B) None.
• 11-D) Glucose and Oxygen.
• 12-C) Makes ATP.
• 13-B) Cells, tissues, systems.
• 14-C) Tissues.

Microscopy and Molecular Biology

• 15- 2) Internal structures: Transmission electron microscope (TEM), 3) 2 more lenses: Compound microscope, 4) 3D image: Scanning electron microscope (SEM), 5) Highest magnification: Scanning tunneling microscope (STM).
• 16-D) Mitch.
• 17-A) Starch molecule.

Steps in Root End Surgery

• Local anesthesia and hemostasis
• Management of soft tissue
• Management of hard tissue
• Surgical access (visual and operative)
• Access to root structure
• Periradicular curettage
• Root end resection
• Root end preparation
• Root end filling
• Soft tissue repositioning and suturing
• Post-surgical care

Root End Preparation

Preparing a cavity to receive root end filling.

According to Carr and Bentkover, this involves a Class I preparation at least 3mm into root dentin with walls parallel to and coincident with the anatomic outline of the pulp space.

Key requirements:

1. The apical 3mm of the root canal must be freshly cleaned and shaped.
2. The preparation must be parallel to and coincident with the anatomic outline of the pulp space.
3. Adequate retention form