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Photosynthesis and Cellular Respiration in Plants

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Most living things obtain their energy either directly or indirectly from the energy of sunlight captured through photosynthesis.

Photosynthesis in 2 Stages

  1. Pigments in the leaves' cells (chlorophyll) capture or absorb the sun's energy, which is used to power the second stage. The leaf cells also need water (absorbed through roots) and carbon dioxide (that enters through small openings on the undersides of leaves called stomata).
  2. Inside the chloroplasts, water and carbon dioxide are transformed into glucose and oxygen. Plants use glucose for food and store what they don't need as starch (energy for later use) and cellulose (that makes up the cell walls). Oxygen and water exit the leaf through the stomata.

Stomata

Carbon dioxide enters the plant... Continue reading "Photosynthesis and Cellular Respiration in Plants" »

Genetic Identification: Restriction Enzymes to DNA Fingerprints

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Understanding DNA Analysis Techniques

Restriction Enzymes: Cutting DNA with Precision

Role: Restriction enzymes cut foreign DNA into pieces to prevent infection.

Mechanism: Special proteins recognize specific DNA sequences (restriction sites) and cut at those locations. Bacteria protect their own DNA from self-cleavage using methylation.

Example: EcoRI

EcoRI recognizes the sequence GAATTC and cuts between G and A. This creates "sticky ends" (overhangs) like G- and AATT, which help join DNA fragments.

DNA Fragmentation and Gel Electrophoresis

DNA is cut into fragments using restriction enzymes. These fragments are then sorted by size using gel electrophoresis.

  • Shorter fragments travel farther and faster in the gel.
  • DNA fragments are stained for visibility,
... Continue reading "Genetic Identification: Restriction Enzymes to DNA Fingerprints" »

Echinoderms & Arthropods: Morphology, Features, and Biology

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Echinoderms

Phylum: Echinodermata

  • Sea stars (starfish)
  • Sea urchins
  • Sand dollars
  • Sea cucumbers
  • Brittle stars

Key Features:

  • Symmetry: Larvae: Bilateral; Adults: Pentaradial
  • Body Covering: Spiny skin, endoskeleton made of calcium carbonate
  • Movement: Tube feet powered by water vascular system
  • Nervous System: No brain; nerve ring with radial nerves
  • Circulation: Water vascular system (no true circulatory system)
  • Respiration: Diffusion via tube feet or skin gills
  • Reproduction: Mostly external fertilization; can regenerate lost parts
  • Habitat: Marine only (no freshwater/terrestrial species)

Water Vascular System

A hydraulic system for movement, feeding, and respiration.

Frequently Asked Questions
  1. What type of symmetry do adult echinoderms have? Pentaradial symmetry
  2. Which
... Continue reading "Echinoderms & Arthropods: Morphology, Features, and Biology" »

Key Biological Systems & Mechanisms Explained

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Frog Embryology: Developmental Stages

Frogs reproduce through external fertilization, where the female lays eggs in water, and the male releases sperm over them. Fertilization occurs in the animal hemisphere of the egg. This process forms a diploid zygote. The point of sperm entry determines the gray crescent, which helps in the later development of the body axis.

1. Cleavage: Early Cell Division

The zygote undergoes holoblastic, unequal, and radial cleavage. The first two cleavages are vertical, forming four equal blastomeres. The third cleavage is horizontal but displaced towards the animal pole, resulting in smaller cells (micromeres) in the animal pole and larger cells (macromeres) in the vegetal pole. These divisions continue to form a morula,... Continue reading "Key Biological Systems & Mechanisms Explained" »

Understanding Human Nutrition: Digestive System Essentials

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Human Nutrition: The Digestive System

Human nutrition involves several key systems: digestive, respiratory, circulatory, and excretory.

Preparation for Digestion

This phase consists of three main parts:

Insalivation

Insalivation involves saliva, a watery substance containing amylase enzymes that break down simple carbohydrates. Saliva is produced in three salivary glands: sublingual, submandibular, and parotid. The tongue, a muscular organ covered in taste buds, helps mix food with saliva, allowing us to taste food.

Mastication

Mastication consists of breaking down food into smaller pieces to ease digestion. Types of teeth include incisors, canines, molars, and premolars. Humans have 20 milk teeth and 32 adult teeth.

Swallowing

Swallowing involves two... Continue reading "Understanding Human Nutrition: Digestive System Essentials" »

Atrial Natriuretic Hormone, Urinalysis & Ovary Function

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Atrial Natriuretic Hormone (ANH)

Atrial natriuretic hormone (ANH) is released by the atria of the heart in response to elevated blood pressure or volume. It lowers blood pressure by encouraging salt excretion in the urine (natriuresis). It also lowers systemic vascular resistance by widening blood vessels.

ANH decreases the kidneys' ability to reabsorb water and salt by blocking the release of renin and aldosterone. To further lessen water retention, it also inhibits the release of antidiuretic hormone (ADH). This combined action reduces blood pressure and volume. By opposing the renin-angiotensin-aldosterone system, ANH preserves fluid equilibrium. It is essential for kidney and heart health. Atrial stretch is what causes the release of ANH.... Continue reading "Atrial Natriuretic Hormone, Urinalysis & Ovary Function" »

Human Body Systems: Excretion, Digestion, and Immunity

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Human Body Systems and Excretion

Tissues/Organs/Organ System

Function in Excretion

Digestive System

Removal of faeces

Respiratory System

Removal of CO2 (alveoli)

Skin (sweat glands)

Removal of sweat and toxins

Urinary System

Blood filtration (Urine)

Absorption vs. Assimilation

Compare and contrast

Absorption and Assimilation

  • Similarity: Both involve nutrients—absorption transfers them into the bloodstream, and assimilation uses them in cells.

  • Difference: Absorption happens in the digestive system; assimilation occurs in tissues.

Digestion vs. Nutrition

Digestion and Nutrition

  • Similarity: Both are essential for energy and growth—digestion breaks down food, and nutrition provides the substances needed for this process.

  • Difference: Digestion is the physical

... Continue reading "Human Body Systems: Excretion, Digestion, and Immunity" »

Kidney Function: Blood Filtration, Urine Formation, and Blood Pressure Regulation

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Renal Blood Filtration: Aorta to Vena Cava

The renal arteries, originating from the abdominal aorta, transport blood to the kidneys. Filtration begins in the glomerulus after these arteries branch into smaller arterioles that supply the nephrons. Bowman's capsule filters waste products, ions, and water from the blood. As the filtrate moves through the renal tubules, waste products are secreted, and essential components are reabsorbed. The remaining filtrate forms urine, which then travels to the ureters. After filtration, deoxygenated blood exits the nephron through venules, enters the renal vein, and is returned to the heart via the inferior vena cava. This intricate process maintains electrolyte and fluid balance.

Urine Production within the

... Continue reading "Kidney Function: Blood Filtration, Urine Formation, and Blood Pressure Regulation" »

Infrared Spectroscopy of Organic Compounds: A Comprehensive Guide

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Infrared Spectra of Alkanes

Alkanes, being saturated hydrocarbons, only contain Carbon-Carbon (C-C) and carbon-hydrogen single bonds. Their IR spectra are relatively simple due to the absence of strong functional groups.

C-H Stretching

The most prominent peaks in an alkane's IR spectrum are due to C-H stretching vibrations. These sharp, strong bands typically appear in the range of 2850-2960 cm-1. The exact wavenumber can vary slightly depending on the specific alkane and its branching.

Infrared Spectra of Alkenes

Alkenes contain at least one carbon-carbon double bond (C=C), along with C-H bonds. The presence of the double bond introduces distinct features in their IR spectra compared to alkanes.

C=C Stretching

The stretching vibration of the C=C... Continue reading "Infrared Spectroscopy of Organic Compounds: A Comprehensive Guide" »

Human Physiology: Muscle, Cardiovascular, and Respiratory Systems

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Muscle Anatomy and Structure

  1. Myofibrils: Rod-like structures inside muscle fibers. Contain:
    • Myosin filaments → A bands (thick)
    • Actin filaments → I bands (thin)
  2. Sarcomere: Functional contractile unit of a muscle. Spans from Z-disc to Z-disc.
  3. Muscle Fiber: Formed by fusion of multiple myoblasts. Multinucleated.
  4. Fasciculus: Bundle of muscle fibers.
  5. Connective Tissue Layers:
    • Endomysium: Surrounds individual muscle fibers.
    • Perimysium: Surrounds fasciculi.
    • Epimysium: Surrounds entire muscle.

Neuromuscular Junction (NMJ)

Synapse between an alpha (α)-motor neuron and a muscle fiber. The membrane is highly invaginated, which increases the surface area for Acetylcholine (ACh) receptors. Calcium channels in the neuron terminal open, leading to vesicles... Continue reading "Human Physiology: Muscle, Cardiovascular, and Respiratory Systems" »