Notes, abstracts, papers, exams and problems of Baccalaureate

Anne Frank's Early Life

Anne Frank was born on June 12, 1929, in Frankfurt, Germany, to a Jewish family. In 1933, her parents moved to Amsterdam, Netherlands.

Fleeing Germany and Hiding

During World War II, the family hid to avoid the Nazis. They left their home and moved to a shelter in the offices of her father's company. It was not a comfortable place, but they had to adapt as it became their new home.

Anne's Life in Hiding

Age and Emotions

Anne was 13 years old. She wrote about the feelings of others, expressing her discomfort and eventual feelings of love.

Protecting from the Nazis

To protect themselves from the Nazis, Anne and her family hid in an apartment where they could not make any noise to avoid being caught.

Anne's Diary

Anne believed that... Continue reading "Anne Frank: Life, Diary, and the Holocaust" »

Gerineldo Analysis and Princess Part

Acid Value Determination in Oils: Principle and Procedure

Here's an explanation of the principle and procedure involved in determining the acid value of an oil sample:

Principle

The acid value of an oil sample is a measure of the amount of free fatty acids present in the oil. The principle of the acid value determination is based on the reaction between the free fatty acids in the oil and a strong base, such as potassium hydroxide (KOH). The reaction is as follows:

RCOOH (Free Fatty Acid) + KOH → RCOOK (Potassium Salt) + H₂O

Procedure

Here's the step-by-step procedure for determining the acid value of an oil sample:

1. Apparatus

• 250 mL Erlenmeyer flask
• Burette
• Pipette
• Volumetric flask
• Phenolphthalein indicator

2. Reagents

• Potassium hydroxide (KOH) solution

Complete the sentences with the verbs below in Past Simple:

walk, enjoy, like, watch, travel, finish, listen

1. Lucy liked her birthday presents.
2. Last weekend I walked in the mountains for 2 hours.
3. We enjoyed the party very much.
4. Ken listened to his favorite CD yesterday.
5. My sister studied Maths for an exam.
6. They traveled to the USA in 2003.
7. Dad washed his car last weekend.
8. The film finished at 11:30.

Write the sentences in negative:

1. Lucy didn't like her birthday presents.
2. Last weekend I didn't walk in the mountains for 2 hours.
3. We didn't enjoy the party very much.
4. Ken didn't listen to his favorite CD yesterday.
5. My sister didn't study months for German.
6. They didn't travel to the USA in 2003.
7. Dad didn't wash his car last weekend.
8. The film didn't finish at 11:30.

Plasmids pBR322 and pUC18/19

pBR322

pBR322, developed in 1977, is a foundational plasmid in molecular biology. Key features include:

• Size: ~4,361 base pairs (bp)
• Origin of Replication: Allows independent replication within bacteria.
• Selection Markers:
  • ampR: Confers ampicillin resistance.
  • tetR: Confers tetracycline resistance.
• Cloning Sites: Multiple sites for inserting foreign DNA.
• Applications: Gene cloning, expression, and manipulation.

pUC18/19

pUC18/19, derived from pBR322 in the early 1980s, simplifies cloning. Key features include:

• Size: ~2,686 bp
• Origin of Replication: High-copy-number pMB1 ori for increased yield.
• Selection Marker:
  • lacZ: Beta-galactosidase gene; enables blue/white screening for insert identification.
• Cloning Sites: Multiple cloning

English Grammar

Verb Tenses

Past Tense Transformations

• Present Simple (eats/eat) → Past Simple (ate)
• Don't/Doesn't → Didn't
• Present Continuous (am, is, are + v ing) → Past Continuous (was, were + v ing)
• Past Simple (-ed or irregular) → Past Perfect (had + past participle)
• Present Perfect (have, has + past participle) → Past Perfect
• Past Perfect (had + past participle) → Past Perfect
• Past Continuous (was, were + v ing) → Past Perfect Continuous (had + been + v ing)
• Present Perfect Continuous → Past Perfect Continuous
• Past Perfect Continuous → Past Perfect Continuous
• Future: Will + infinitive → Would + infinitive
• Can → Could + infinitive
• Must/Have to → Had to + infinitive
• May → Might + infinitive

Changes in Reported Speech

• Now → Then
• Today

Distinctive Feature Theory

Distinctive Feature Theory challenges Phoneme Theory (PT) by focusing on the features that compose phonemes, rather than the phonemes themselves. This approach questions the validity of the phoneme concept, arguing that speech is not simply a series of discrete sounds.

Natural Class: A set of sounds sharing phonetic features, affected by the same environment, and having the same effect on surrounding sounds.

Major Class Features

• Vowels: [+SYLLABIC], [+SONORANT], [-CONSONANTAL] - form the syllable nucleus.
• Glides: [-SYLLABIC], [+SONORANT], [-CONSONANTAL].
• Sonorant Consonants (nasals and liquids): [+/-SYLLABIC], [+SONORANT], [+CONSONANTAL].
• Obstruents: [-SYLLABIC], [-SONORANT], [+CONSONANTAL].

Consonant Features

• Voice: [+/-VOICE]